This kit uses double antigen sandwich colloidal gold immunochromatography to detect SARS-CoV-2 neutralizing antibodies in human serum/ plasma and whole blood. The membrane was precoated with SARS-CoV-2 S-RBD protein on the test zone and pre-coated goat anti-chicken IgY on the control zone. During detection, the colloidal gold labeled SARS-CoV-2 S-RBD proteins bind to the SARS-CoV-2 neutralizing antibodies in the sample to form immune complexes. The immune complexes move forward along the cassette by chromatography, then captured by SARS-CoV-2 S-RBD proteins on the test zone and produce a visual red color line. The color of line is positive correlated with the amount of SARS-CoV-2 neutralizing antibodies in specimen. The colloidal gold-labeled chicken IgY antibodies are combined with the pre-coated goat anti-chicken IgY antibodies on the quality control line to form the quality control line C.