Cardiac Troponin-I (cTnI) quantitative rapid test kit, is a double antibody sandwich immunoassay kit for the quantitative testing of cTnI in serum, plasma and whole blood. The membrane was precoated with cTnI specificity antibodies on the test band region and Goat anti-mouse IgG antibody on the control band region. During the test, the specimen is allowed to react with matched cTnI Specific antibodies -colloid gold conjugate, which was predried on the test strip. The conjugate binds to the cTnI forming an antibody-antigen complex. The complex moves forward on the membrane by the capillary action, then the complex is captured by cTnI Specific antibodies on the test region to produce a visual pink color band. Regardless of the presence of cTnI, as the mixture continues to move across the membrane to the control band region, the complex is captured by immobilized Goat anti-mouse IgG antibodies to form a distinct pink colored control band. Coordinate with the Standard curve line of Rapid Immuno Assay Reader to read the content of cTnI.