Antibodies to LH are precoated onto membrane as capture reagent on the test zone. During the test, specimen is allowed to react with the colloidal gold particles, which have been labeled with antibodies to LH. If LH is present, a red colored line will develop on the membrane in proportion to the amount of LH present in the specimen. When test line is similar to or darker than control line indicated that there is a LH surge. When test line is lighter than control line, showing LH content is not in the peaking. Only control line is colored, showing LH level is very low. To serve as a procedural control, red colored line in the control zone will always appear regardless of the presence of LH.