This kit uses double antibody sandwich immunoassay to detect specific antigens to Dengue NS1 in human serum, plasma or whole blood. The membrane was precoated with Dengue NS1 specific antibody on the test zone and goat anti mouse IgG antibody on the control zone. During the test, the sample is allowed to react with Dengue NS1 specific antibody-colloidal gold particles conjugate. The conjugate binds to the Dengue NS1 forming an antibody ~ antigen complex. The complex moves forward on the membrane by the capillary action, then the complex is captured by specific antibody to Dengue NS1 on the test zone to produce a visual color band. The color of band is positive correlated with the amount of Dengue NS1 in sample. Regardless of the presence of Dengue NS1, as the mixture continues to move across the membrane to the control zone, the complex is captured by immobilized goat anti mouse IgG antibody to form a distinct color band.