This kit uses indirect immunocolloidal gold technology to detect the TB IgG antibody in samples. The colloidal gold pad of the reagent contained the colloidal gold particles labeled with monoclonal antibody mouse anti-human (IgG1 subtype). The T-zone of NC membrane is coated with recombinant expression of mycobacterium tuberculosis 38KD and 16KD antigen, and the C-zone is coated with goat anti-mouse IgG polyclonal antibody. During the testing, When labeled colloidal gold chromatography to the T zone, if there is human IgG antibody bound to the T zone, it will bind with the colloidal gold to form a mouse anti-human labeled colloidal gold-tuberculosis specific IgG-tuberculosis specific antigen complex, and present a red colloidal gold line on the T zone. If there is no binding human IgG antibody on the T zone, it will not form complex and the T zone does not show color. When the labeled colloidal gold passes through the C zone, it will combine with the goat anti-mouse IgG to form the colloidal gold-goat anti-mouse IgG complex labeled with mouse anti-human, and present a red colloidal gold line on the C zone. If the C zone does not show color, it indicates that there is a problem in the test, and the result is invalid, it should be retried. This kit adopts the detection method of adding samples on the membrane, which can avoid the Hook effect caused by too much non-specific IgG in samples.