HIV-1 (gp41 and gp120) and HIV-2 (gp36) specific recombinant antigens are separately precoated onto the membrane in zone 1 and 2 as the capture reagent on the test zone. During the test, specimen is allowed to react with the colloidal gold particles, which have been conjugated with HIV-1 and HIV-2 specific recombinant antigens. Antibodies to HIV-1 and/ or HIV-2, if present, will specifically bind to colloidal gold-antigen complex. When the colloidal gold-antigen-antibody complexes move to the test zone, they will specifically bind to the precoated antigens. At the same time, a red colored line will develop in zone 1 and/ or 2 on the membrane. Absence of these red colored lines in the test zone (1 and 2) suggests a negative result. To serve as a procedural control, red colored line in the control zone will always appear regardless of the presence of antibodies to HIV-1/ HIV-2.